Product Description
Type V Collagen provides structure and strength to connective tissue and is found in many places in the body, especially skin, lung, intestinal walls and walls of blood vessels. This Type V Collagen product is isolated from placenta and is purified using a multi-step process with a final purity of >95%. Type V collagen forms a triple helix composed of aAlpha1(V)2 Alpha 2(V) heterotrimer.
The product is supplied as a sterile solution at 0.5 mg per vial at approximately 0.15 to 0.30 mg/ml in 100 mM acetic acid. A Certificate of Analysis is available with the purchase of each product. Type V collagen product is provided in a user-friendly packaging for use and storage.
Type V Collagen is typically used as a thin coating on tissue culture surfaces. Specific instructions are found in the Directions for Use. This product is generally usedin vitroas a substrate scaffold to enhance cell attachment, adherence and proliferation. Type V collagen may be used to culture a variety of cell types.
| Parameter, Testing, and Method | Type V Collagen#5168 |
| Sterilization Method | Filtration |
| Form | Solution |
| Package Size | 0.5 mg (~2 mL) |
| Storage Temperature | 2-10°C |
| Shelf Life | Minimum of 6 months from date of receipt |
Collagen Concentration - Biuret | 0.15-0.3 mg/mL |
| pH | 2.0-3.5 |
Electrophoretic Pattern - Coomassie Blue | Characteristic |
| Endotoxin - LAL | <5.0 EU/mL |
| Cell Attachment Assay | Pass |
| Source | Bovine Placenta |
Directions for Use
Download the full PDF versionor continue reading below:
Coating Procedure
Note:Use these recommendations as guidelines todetermine the optimal coating conditions for your culturesystem.
- Remove required quantity of collagen from the bottle and dispense into a dilution vessel.
- Dilute Type V Collagen with 100 mM acetic acid to ~10 µg/ml.
- Swirl contents gently until material is completelymixed.
- Add appropriate amount of diluted Type V Collagenmaterial to the culture surface ensuring that the entire surface is coated.
- Incubate at room temperature, covered, for 1-2 hours.
- After incubation, aspirate any remaining material.
- Rinse coated surfaces carefully with sterile medium orPBS, avoid scratching surfaces.
- Coated surfaces are ready for use. They may also bestored at 2-8°C damp or air dried if sterility is maintained.
Product Certificate of Analysis
Product Videos
Coating Plates with Collagen
Video
Coating a Glass Coverslip with Collagen
Video
See More
Safety and Documentation
Safety Data Sheet
Certificate of Origin
Product Disclaimer
This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
Advanced BioMatrix（简称ABM）是美国一家著名的生物公司，获得了Allergan Inc的授权(Allergan用25年时间不断完善胶原蛋白相关的产品的生产工艺)，将Allergan的专业和技术用于蛋白生产与检测，致力于为组织工程、细胞分析及细胞增殖等研究领域提供优质稳定的产品。
Advanced BioMatrix不断丰富已有产品线，目前可为三维细胞培养提供各种胶原蛋白、纤连蛋白、玻连蛋白、水性凝胶、不同粘度与分子量的透明质酸以及低代成纤维细胞等。在美国全部产品授权Sigma销售。
Advanced BioMatrix是组织培养，细胞分析和细胞增殖三维（3D）应用的生命科学领域的领导者。 我们的产品被公认为纯度，功能性和一致性的标准。
我们在生产，分离，纯化，冷冻干燥，细胞培养和蛋白质测试，粘附肽，附着因子，底物刚性和其他3D矩阵产品方面拥有丰富的专业知识。 我们的专业技术和知识正在被用来确保我们的产品质量最高，批次之间一致且易于为我们的研究客户使用。
本网站将在规定时间内给予删除等相关处理。
