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胶原蛋白、纤连蛋白、玻连蛋白、水性凝胶、低代成纤维细胞
℡ 4000-520-616
℡ 4000-520-616
Advanced BioMatrix/PhotoCol®-IRG//5201-1KIT
产品编号:5201-1KIT
市  场 价:¥6100.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$305.00
品      牌: Advanced BioMatrix
公      司:Advanced BioMatrix ,Inc.
公司分类:
Advanced BioMatrix/PhotoCol®-IRG//5201-1KIT
商品介绍

Product Description

Advanced BioMatrix offers PhotoCol®, a purified methacrylated Type I bovine collagen kit. PhotoCol® provides native-like 3D collagen gels with the unique attributes to be prepared at various concentrations and crosslinked to provide various gel stiffness.

The PhotoCol® kit consists of purified methacrylated Type I bovine collagen as the core component with other support reagents in the kit. Table 1 provides a list of the kit components.

Table 1:

ItemCatalog NumberPackage SizeStorage Temperature
Methacrylated Collagen#5198100 mg2-10°C
20 mM Acetic Acid#507950 mL2-30°C
Neutralization Solution#520510 mLRoom Temperature
Irgacure Photoinitiator#5200100 mg2-10°C

The methacrylated Type I collagen is produced from telo-peptide intact bovine collagen where the collagen has been modified by reacting the free amines, primarily the ε-amines groups of the lysine residues as well as the a-amines groups on theN-termini. > 20% of the total lysine residues of the collagen molecule have been methacrylated. The collagen is extracted from bovine hide and contains a high monomer content.

The collagen starting material was isolated from a closed herd and purified using controlled manufacturing processes.A bottle of 20 mM acetic acid solution is provided to solubilize the lyophilized methacrylated collagen at concentrations ranging from 3 to 8 mg/ml. The neutralization solution consists of an alkaline 10X phosphate buffered saline (PBS) solution which provides physiological salts and pH in the final mixture. The photoinitiator consists of Irgacure 2959 to be formulated in methanol (methanol not included) which allows UV crosslinking of the collagen at 365 nm.

Parameter, Testing, and MethodMethacrylated Collagen#5198
Sterilization MethodFiltration
Extraction MethodAcid - Telocollagen
FormLyophilized Powder
Degree of Methacrylation>20%
Package Size100 mg
Shelf LifeMinimum of 6 months from date of receipt
Shelf Life After Reconstitution2 Months

Collagen Purity - Silver Staining

>99%
Kinetic Gel Test (Minutes)<40
Gel Formation Tube Test (Minutes)< 40

Electrophoretic Pattern - Coomassie Blue

Characteristic
Sterility - USP modifiedNo growth
Endotoxin - LAL<10.0 EU/mL
SourceBovine Hide
Hydrogel Young's Modulus E (Pa)Characteristic

Directions for Use

Download the full Directions for Use PDF

Product Applications

PhotoCol®Methacrylated Collagen is a photocrosslinkable type I collagen for 3D cell culture and bioprinting.

As the most abundant protein in the body, collagen is a key component for cell culture. Collagen methacrylate can be used as a rapidly self-assembling type I collagen to form cross-linked hydrogels for tissue engineering[1]. It has been used with mesenchymal stem cells[2], fibroblasts, adipose derived stem cells, epithelial cells, and many more.

Collagen methacrylate is useful for forming scaffolds with varying degree of stiffness, by altering collagen concentration or the dose of UV-light exposure[2]. It is also being extensively characterized for its usefulness in 3D bioprinting (extrusion, inkjet, and photolithographic[3]).

PhotoCol® has been used for created 3D microniches[4], as well as for 3D bioprinting human corneas[5].

References:

  1. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4172302/

  2. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4243547/

  3. http://www.worldscientific.com/doi/abs/10.1142/S2339547817500091

  4. https://onlinelibrary.wiley.com/doi/abs/10.1002/adbi.201700237

  5. https://www.ncbi.nlm.nih.gov/pubmed/29772228

Product References

References for PhotoCol®:

Isaacson, A., Swioklo, S. & Connon, C. J. 3D bioprinting of a corneal stroma equivalent.Experimental Eye Research173,188–193 (2018).

Kathryn E. Drzewiecki et al, A thermoreversible, photocrosslinkable collagen bio-ink for free-form fabrication of scaffolds for regenerative medicine,TECHNOLOGY(2017).

Drzewiecki, K. E.et al.Methacrylation Induces Rapid, Temperature-Dependent, Reversible Self-Assembly of Type-I Collagen.Langmuir30,11204–11211 (2014).

Gaudet, I. D. & Shreiber, D. I. Characterization of Methacrylated Type-I Collagen as a Dynamic, Photoactive Hydrogel.Biointerphases7,25 (2012).

Izadifar, Mohammad, et al. "UV-assisted 3D bioprinting of nanoreinforced hybrid cardiac patch for myocardial tissue engineering."Tissue Engineering Part C: Methods24.2 (2018): 74-88.

Stoecklin, Celine, et al. "A New Approach to Design Artificial 3D Microniches with Combined Chemical, Topographical, and Rheological Cues."Advanced Biosystems(2018): 1700237.

Mazzocchi, Andrea, et al. "Optimization of collagen type I-hyaluronan hybrid bioink for 3D bioprinted liver microenvironments."Biofabrication(2018).

Nguyen, T. U., Watkins, K. E. & Kishore, V. Photochemically crosslinked cell‐laden methacrylated collagen hydrogels with high cell viability and functionality.Journal of Biomedical Materials Research Part A107,1541–1550 (2019).

Mazzocchi, A.et al.Pleural Effusion Aspirate for Use in 3D Lung Cancer Modeling and Chemotherapy Screening.ACS Biomaterials Science & Engineering5,1937–1943 (2019).

Chhetri, A.et al.Cell Culture and Coculture for Oncological Research in Appropriate Microenvironments.Current Protocols in Chemical Biology11,(2019).

Product Certificate of Analysis

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Product Videos

link to library blog - How to Make 3D Collagen Hydrogels
How to Make 3D Collagen Hydrogels

Video

link to library blog - Telo vs Atelo Collagen
Telo vs Atelo Collagen

Video

link to library blog - Neutralization Solution for Easy 3D Collagen Gels
Neutralization Solution for Easy 3D Collagen Gels

Video

link to library blog - Seeding Collagen Gels with Cells
Seeding Collagen Gels with Cells

Video

link to library blog - 4 Key Components for 3D Collagen Gels
4 Key Components for 3D Collagen Gels

Video

See More

Safety and Documentation

Safety Data Sheet

Certificate of Origin

Declaration of Material Source

Product Disclaimer

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

品牌介绍
美国Advanced BioMatrix(简称ABM) www.advancedbiomatrix.com
Advanced BioMatrix(简称ABM)是美国一家著名的生物公司,获得了Allergan Inc的授权(Allergan用25年时间不断完善胶原蛋白相关的产品的生产工艺),将Allergan的专业和技术用于蛋白生产与检测,致力于为组织工程、细胞分析及细胞增殖等研究领域提供优质稳定的产品。

Advanced BioMatrix不断丰富已有产品线,目前可为三维细胞培养提供各种胶原蛋白、纤连蛋白、玻连蛋白、水性凝胶、不同粘度与分子量的透明质酸以及低代成纤维细胞等。在美国全部产品授权Sigma销售。

Advanced BioMatrix是组织培养,细胞分析和细胞增殖三维(3D)应用的生命科学领域的领导者。 我们的产品被公认为纯度,功能性和一致性的标准。
我们在生产,分离,纯化,冷冻干燥,细胞培养和蛋白质测试,粘附肽,附着因子,底物刚性和其他3D矩阵产品方面拥有丰富的专业知识。 我们的专业技术和知识正在被用来确保我们的产品质量最高,批次之间一致且易于为我们的研究客户使用。

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