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胶原蛋白、纤连蛋白、玻连蛋白、水性凝胶、低代成纤维细胞
℡ 4000-520-616
℡ 4000-520-616
Advanced BioMatrix/PhotoCol®-RUT//5271-1KIT
产品编号:5271-1KIT
市  场 价:¥6100.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$305.00
品      牌: Advanced BioMatrix
公      司:Advanced BioMatrix ,Inc.
公司分类:
Advanced BioMatrix/PhotoCol®-RUT//5271-1KIT
商品介绍

ProductDescription

AdvancedBiomatrixoffersPhotoCol®,apurifiedmethacrylatedTypeIbovinecollagenkit.PhotoCol®providesnative-like3Dcollagengelswiththeuniqueattributestobepreparedatvariousconcentrationsandcrosslinkedtoprovidevariousgelstiffness.ThePhotoCol®kitconsistsofpurifiedmethacrylatedTypeIbovinecollagenasthecorecomponentwithothersupportreagentsinthekit.Table1providesalistofthekitcomponents.

ItemCatalogNumberPackageSizeStorageTemperature
MethacrylatedCollagen#5198100mg2-10°C
20mMAceticAcid#507950mL2-30°C
NeutralizationSolution#520510mLRoomTemperature
RutheniumPhotoinitiator#5246100mgRoomTemperature
SodiumPersulfatePhotoinitiator#5247500mgRoomTemperature

ThemethacrylatedTypeIcollagenisproducedfromtelo-peptideintactbovinecollagenwherethecollagenhasbeenmodifiedbyreactingthefreeamines,primarilytheε-aminesgroupsofthelysineresiduesaswellasthea-aminesgroupsontheN-termini.>20%ofthetotallysineresiduesofthecollagenmoleculehavebeenmethacrylated.Thecollagenisextractedfrombovinehideandcontainsahighmonomercontent.

Thecollagenstartingmaterialwasisolatedfromaclosedherdandpurifiedusingcontrolledmanufacturingprocesses.Abottleof20mMaceticacidsolutionisprovidedtosolubilizethelyophilizedmethacrylatedcollagenatconcentrationsrangingfrom3to8mg/ml.Theneutralizationsolutionconsistsofanalkaline10Xphosphatebufferedsaline(PBS)solutionwhichprovidesphysiologicalsaltsandpHinthefinalmixture.

ThephotoinitiatorconsistsofRutheniumandSodiumPersulfatetobeformulatedin1xPBSorcellculturemediawhichallowsvisIBLelightphotocrosslinkingofthecollagenat400-450nm.Werecommendusingantibioticswiththephotoinitiator,orfilteringthrougha0.2micronbuttonfilterforcellculturework.

Parameter,Testing,andMethodMethacrylatedCollagen#5198
SterilizationMethodFiltration
ExtractionMethodAcid-Telocollagen
FormLyophilizedPowder
PackageSize100mg
DegreeofMethacrylation>20%
StorageTemperature2-10°C
ShelfLifeMinimumof6monthsfromdateofreceipt
ShelfLifeAfterReconstitution2Months

CollagenPurity-SilverStaining

>99%
KineticGelTest(Minutes)<40

GelFormationTubeTest(Minutes)

<40

ElectrophoreticPattern-CoomassieBlue

Characteristic
Sterility-USPmodifiedNogrowth
Endotoxin(LAL)<10.0EU/mL
SourceBovineHide
HydrogelYoung"sModulusE(Pa)Characteristic

DirectionsforUse

DownloadthefullDirectionsforUsePDF

ProductApplications

PhotoCol®MethacrylatedCollagenisaphotocrosslinkabletypeIcollagenfor3Dcellcultureandbioprinting.

Asthemostabundantproteininthebody,collagenisakeycomponentforcellculture.Collagenmethacrylatecanbeusedasarapidlyself-assemblingtypeIcollagentoformcross-linkedhydrogelsfortissueengineering[1].Ithasbeenusedwithmesenchymalstemcells[2],fibroblasts,ADIposederivedstemcells,epithelialcells,andmanymore.

Collagenmethacrylateisusefulforformingscaffoldswithvaryingdegreeofstiffness,byalteringcollagenconcentrationorthedoseofUV-lightexposure[2].Itisalsobeingextensivelycharacterizedforitsusefulnessin3Dbioprinting(extrusion,inkjet,andphotolithographic[3]).

PhotoCol®hasbeenusedforcreated3Dmicroniches[4],aswellasfor3Dbioprintinghumancorneas[5].

References:

  1. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4172302/

  2. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4243547/

  3. http://www.worldscientific.com/doi/abs/10.1142/S2339547817500091

  4. https://onlinelibrary.wiley.com/doi/abs/10.1002/adbi.201700237

  5. https://www.ncbi.nlm.nih.gov/pubmed/29772228

ProductReferences

ReferencesforPhotoCol®:

Isaacson,A.,Swioklo,S.&Connon,C.J.3Dbioprintingofacornealstromaequivalent.ExperimentalEyeResearch173,188–193(2018).

KathrynE.Drzewieckietal,AThermoreversible,photocrosslinkablecollagenbio-inkforfree-formfabricationofscaffoldsforregenerativemedicine,TECHNOLOGY(2017).

Drzewiecki,K.E.etal.MethacrylationInducesRapid,Temperature-Dependent,ReversibleSelf-AssemblyofType-ICollagen.Langmuir30,11204–11211(2014).

Gaudet,I.D.&Shreiber,D.I.CharacterizationofMethacrylatedType-ICollagenasaDynamic,PhotoactiveHydrogel.Biointerphases7,25(2012).

Izadifar,Mohammad,etal."UV-assisted3Dbioprintingofnanoreinforcedhybridcardiacpatchformyocardialtissueengineering."TissueEngineeringPartC:Methods24.2(2018):74-88.

Stoecklin,Celine,etal."ANewApproachtoDesignArtificial3DMicronicheswithCombinedChemical,Topographical,andRheologicalCues."AdvancedBiosystems(2018):1700237.

Mazzocchi,Andrea,etal."OptimizationofcollagentypeI-hyaluronanhybridbioinkfor3Dbioprintedlivermicroenvironments."Biofabrication(2018).

Nguyen,T.U.,Watkins,K.E.&Kishore,V.Photochemicallycrosslinkedcell‐ladenmethacrylatedcollagenhydrogelswithhighcellviABIlityandfunctionality.JournalofBiomedicalMaterialsResearchPartA107,1541–1550(2019).

Mazzocchi,A.etal.PleuralEffusionAspirateforUsein3DLungCancerModelingandChemotherapyScreening.ACSBiomaterialsScience&Engineering5,1937–1943(2019).

Chhetri,A.etal.CellCultureandCocultureforOncologicalResearchinAppropriateMicroenvironments.CurrentProtocolsinChemicalBIOLOGy11,(2019).

ProductCertificateofAnalysis

Noresultfor.

ProductVideos

link to library blog - How to Make 3D Collagen Hydrogels
HowtoMake3DCollagenHydrogels

Video

link to library blog - Telo vs Atelo Collagen
TelovsAteloCollagen

Video

link to library blog - Seeding Collagen Gels with Cells
SeedingCollagenGelswithCells

Video

link to library blog - Neutralization Solution for Easy 3D Collagen Gels
NeutralizationSolutionforEasy3DCollagenGels

Video

link to library blog - 4 Key Components for 3D Collagen Gels
4KeyComponentsfor3DCollagenGels

Video

SeeMore

SafetyandDocumentation

SafetyDataSheet

CertificateofOrigin

DeclarationofMaterialSource

ProductDisclaimer

ThisproductisforR&Duseonlyandisnotintendedforhumanorotheruses.PleaseconsulttheMaterialSafetyDataSheetforinformationregardinghazardsandsafehandlingpractices.

品牌介绍
美国Advanced BioMatrix(简称ABM) www.advancedbiomatrix.com
Advanced BioMatrix(简称ABM)是美国一家著名的生物公司,获得了Allergan Inc的授权(Allergan用25年时间不断完善胶原蛋白相关的产品的生产工艺),将Allergan的专业和技术用于蛋白生产与检测,致力于为组织工程、细胞分析及细胞增殖等研究领域提供优质稳定的产品。

Advanced BioMatrix不断丰富已有产品线,目前可为三维细胞培养提供各种胶原蛋白、纤连蛋白、玻连蛋白、水性凝胶、不同粘度与分子量的透明质酸以及低代成纤维细胞等。在美国全部产品授权Sigma销售。

Advanced BioMatrix是组织培养,细胞分析和细胞增殖三维(3D)应用的生命科学领域的领导者。 我们的产品被公认为纯度,功能性和一致性的标准。
我们在生产,分离,纯化,冷冻干燥,细胞培养和蛋白质测试,粘附肽,附着因子,底物刚性和其他3D矩阵产品方面拥有丰富的专业知识。 我们的专业技术和知识正在被用来确保我们的产品质量最高,批次之间一致且易于为我们的研究客户使用。

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