ProductDescription
PolyethyleneGlycolDiacrylate(PEGDA)hydrogelsarepowerfultoolsforuncoveringbasiccellularBIOLOGybecausetheyareconsideredbiologicallyinert(“blankslate”)andtheirmechanicalpropertiescanbevariedoveralargerangeofmoduli.
PEGDAisanemergingscaffoldfortissueengineeringandregenerativemedicinesincepolymerizationcanoccurrapidlyatroomtemperatureandrequireslowenergyinput,hashighwatercontent,iselastic,andcanbecustomizedtoincludeavarietyofbiologicalmolecules.
PEGDAisacrosslinkerrepeatingpolymercrosslinkerandis>80%acrylated.Itcanbeusedasathiol-reactivecrosslinkerinourHyStemsystemsatlowconcentration,orcanbeusedtomakeaPEGDA-onlyhydrogelinthepresenceofafree-rADIcalchainphotoinitiatorandlightsource.PEG-onlygelsdonottypicallysupportcellattachmentwithouttheincorporationofcellularattachmentsites.ApplicationsRegularand3-DcellcultureTissueengineeringPhotolithography
StorePEGDAintheoriginalvialunopenedat-20°Cforuptooneyear.ReconstitutedPEGDAsolutionscanbestoredat-20°Cfor~onemonth.
DirectionsforUse
DownloadthefullDirectionsforUsePDFhereorcontinuereadingbelow:
PEGDAispreparedbydissolvingthelyophilizedsolidintheDGWater.PEGDAhydrogels(1mL)shouldbepreparedinthefollowingmanner:
- AllowthePEGDAtocometoroomtemperature.
- Underasepticconditionsadd1.0mLofDGWaterto100mgofPEGDAvialfora10%(w/v)solution.
- Invertseveraltimestodissolve.Thesolutionwillbeclearandslightlyviscous.
- Tosterilize,filterthrougha0.2micronfilter.
- AdddesiredvolumeofphotoinitiatortoPEGDA.Placethevialonarockerorshakerandallowsolidstocompletelydissolve(approximately10-20minutes).
- Keepsolutionprotectedfromlightuntilreadytocrosslink.Pipettesolutionintodesiredformat(i.e.96wellplate)andphotocrosslink.
- Hydrogelpropertiesmayvarydependingontimeofexposureandtypeoflight.EnsurethePhotocrosslinkinglightisincloseproximitytothehydrogelsolution.
- FreezeunusedPEGDAsolutionat-20°Candprotectedfromlight.
ProductApplications
Clickonthetitleofthedesiredprotocoltolearnmore:
2DCellGrowthonHyStemHydrogels
HyStem3DCellEncapsulationforCellDeliveryApplicationsGuide
HyStem3DCellEncapsulationinhydrogelsusing96-wellplates
HyStem3DCellEncapsulationinhydrogelsusingTCInserts
EnzymeDigestionofHyStemHydrogelsforRecoveryofEncapsulatedCells
FluorescentLabelingofHyStemHydrogels
CellRecoveryfromSurfaceofHyStemHydrogels
HyStemECMIncorporation
HyStemGelationTimeVariation
HyStemStiffnessVariationProtocolfor7.5mLkit
HyStemStiffnessVariationProtocolfor12.5mLkit
ProductCertificateofAnalysis
SafetyandDocumentation
CertificateofOrigin
SafetyDataSheet
ProductDisclaimer
ThisproductisforR&Duseonlyandisnotintendedforhumanorotheruses.PleaseconsulttheMaterialSafetyDataSheetforinformationregardinghazardsandsafehandlingpractices.
Advanced BioMatrix（简称ABM）是美国一家著名的生物公司，获得了Allergan Inc的授权(Allergan用25年时间不断完善胶原蛋白相关的产品的生产工艺)，将Allergan的专业和技术用于蛋白生产与检测，致力于为组织工程、细胞分析及细胞增殖等研究领域提供优质稳定的产品。
Advanced BioMatrix不断丰富已有产品线，目前可为三维细胞培养提供各种胶原蛋白、纤连蛋白、玻连蛋白、水性凝胶、不同粘度与分子量的透明质酸以及低代成纤维细胞等。在美国全部产品授权Sigma销售。
Advanced BioMatrix是组织培养，细胞分析和细胞增殖三维（3D）应用的生命科学领域的领导者。 我们的产品被公认为纯度，功能性和一致性的标准。
我们在生产，分离，纯化，冷冻干燥，细胞培养和蛋白质测试，粘附肽，附着因子，底物刚性和其他3D矩阵产品方面拥有丰富的专业知识。 我们的专业技术和知识正在被用来确保我们的产品质量最高，批次之间一致且易于为我们的研究客户使用。
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