ProductDescription
Mebiol®ThermoreversIBLeHydrogelis10gramsofacopolymerofpoly(N-isopropylacrylamide)andpoly(ethyleneglycol)(PNIPAAm-PEG).ThedefiningfeatureofMebiol®isitstemperaturereversiblesol-geltransition.ChillMebiol®toturnitintoasol(meaningithandleslikealiquid).HeatMebiol®toturnitintoasolidhydrogel.Theuniquethermoreversiblefeatureallowsforextremelyeasycellhandling.CulturesareseededintocooledMebiol®andrecoveredconvenientlybycoolingtheculturevesselandcentrifugation.Inthegelstate,thehighlylipophilicandnetworkstructureoftheMebiol®presentsanefficientnicheforcellproliferation,cellcommunication,gasandmassexchange,andprotectionofcellsandtissuefromshearforces.
Mebiol®iseasytouse,non-toxic,100%syntheticandhashightransparency.Publishedapplicationsincludestemcellandpluripotentstemcellculture,expansion,anddifferentiation,spheroidculture,cellimplantation,organandtissueregeneration,drugdeliveryandmore.ThoughMebiol®suppressesfibroblastgrowth,ithasbeencommercializedasacell/tissueculturereagentforEScells,chondrocytesandcancercells.
| Parameter,Testing,andMethod | Mebiol®ThermoreversibleHydrogel#5180 |
| PackageSize | 10mL |
| Form | Lyophilized |
| StorageTemperatures | Roomtemperaturepriortosolubilization.Aftersolubilization,storeat2-8°Cfor1monthoraliquotat-20°Cforlongtermstorage |
| FluidTemperature | 0-15°C |
| GelTemperature | >25°C |
| SterilizationMethod | EthyleneOxide |
| ShelfLife | Minimumof6monthsfromdateofreceipt |
DirectionsforUse
DownloadthefullPDFversionorcontinuereADIngbelow:
CoatingProcedure
CellcultureinMebiol®flask:
- Openpackageinsterileenvironmentandadd10mLofculturemediadirectlytoMebiol®flask.
- Closeflaskandlayinrefrigerator(2-10°C)for3hourstoallowMebiol®toabsorbmedia.
- PeriodicallyshakeflaskgentlytohelpdissolvetheMebiol®intothemedia.Maketakeuptoadayforcompletedissolution(maintainatcoldtemperature).
- Afterdissolving,allowtositinrefrigeratoruntilbubblesareeliminated.
- Addcells/tissuesintoMebiol®atlowtemperature(2-10°C)andwarmto37°Ctoculturecells/tissuesina3Dhydrogelenvironment.
- Torecoverthecells/tissues,cooltheMebiol®backdownanddilutewith30-40mLofcoldsalineormedium.Thiswilltakeitoutofgelphase.SUSPendedcellscanbeeasilyrecoveredviacentrifugation.
Cellculturein24-wellplate:
- Followsteps1-4above.
- Beforeaddingcells/tissues,removedesiredamountofMebiol®andputintochilledcentrifugetube.PutremainingMebiol®intorefrigerator(stillinoriginalflask).
- Addapproximately105cell/mLtochilledcentrifugetubeandmixgentlyonice.
- Warmup24-wellplateandseparateoverlayingculturemediato37°C.
- Add200µLofcoldMebiol®suspensiontothecenterofeachwell.MakesureMebiol®doesnotcoverentirebottomofthewelltomakeiteasiertochangeoverlaidmediuminlaterstep.
- Overlay400µLofwarmedculturemediacontainingphenolredonMebiol®suspension.Cultureat37°C.
- Torecovercells,refrigerateat2-8°Canddilutewith400µLofcoldsalineormedium.ThiswilltakeMebiol®outofgelphase.Suspendedcellscanbeeasilyrecoveredviacentrifugation.
Donotre-sterilizetoavoiddeterioration.Addmediapromptlyafteropening,storeinrefrigerationandusewithin1month.
ProductQ&A
Thereisawiderangeofmolecularweightdistribution,butthemolecularweightisatleast>100,000.
PhysicalPropertiesAsynthetichydrogelwiththermoreversiblegelation.II.:Effectofaddedsalts.H.Yoshioka,M.Mikami,Y.Mori,andE.Tsuchida.J.Macromol.Sci.,A31(1),121-125(1994).http://www.tandfonline.com/doi/abs/10.1080/10601329409349723#.UvFzqtKE1NQ
PhysicalPropertiesThermoreversiblegelationonheatingandoncoolingofanaqueousgelatin-poly(N-isopropylacrylamide)conjugate.,H.Yoshioka,Y.Mori,S.Tsukikawa,andS.Kubota,Polym.Adv.Tech.,9,155-158(1998).http://onlinelibrary.wiley.com/doi/10.1002/(SICI)1099-1581(199802)9:2%3C155::AID-PAT750%3E3.0.CO;2-G/abstract
PhysicalPropertiesASynthetichydrogelwiththermoreversiblegelation.I.Preparationandrheologicalproperties,H.Yoshioka,M.MikamiandY.Mori.J.M.S-PureAppl.Chem.,A31(1),pp.113-120(1994).http://www.tandfonline.com/doi/abs/10.1080/10601329409349722#.UvFK1dKE1NQ
PhysicalPropertiesPreparationofPoly(N-Isopropylacrylamide)-b-Poly(EthyleneGlycol)andCalorimetricAnalysisofitsAqueousSolution,H.Yoshioka,M.MikamiandY.Mori,J.M.S-PureAppl.Chem.,A31(1),pp.109-112(1994).http://www.tandfonline.com/doi/abs/10.1080/10601329409349721#.UvFLB9KE1NQ
PhysicalPropertiesAsynthetichydrogelwiththermoreversiblegelation.III.:AnNMRstudyoftheSol-Geltransition.H.Yoshioka,Y.MoriandJamesA.Cushman.Polym.Adv.Tech.,5,pp.122-127(1994).http://onlinelibrary.wiley.com/doi/10.1002/pat.1994.220050207/abstract
ProductReferences
Mebiol®References:
Aeby,EliseA.,etal."MicrofluidicHydrogelHanging‐DropNetworkforLong‐TermCulturingof3DMicrotissuesandSimultaneousHigh‐ResolutionImaging."AdvancedBiosystems2.7(2018):1800054.
Sudha,B.,etal."CultivationofhumancorneallimbalstemcellsinMebiolgel®-Athermo-reversiblegelationpolymer."IndianJournalofMedicalResearch124.6(2006):655.
Sitalakshmi,G.,etal."Exvivocultivationofcorneallimbalepithelialcellsinathermoreversiblepolymer(MebiolGel)andtheirtransplantationinrabbits:ananimalmodel."TissueEngineeringPartA15.2(2008):407-415.
Kataoka,Ken,andNam-hoHuh."Applicationofathermo-reversiblegelationpolymer,mebiolgel,forstemcellcultureandregenerativemedicine."Journalofstemcells®enerativemedicine6.1(2010):10.
Madhavan,H.N.,etal."AstudyonthegrowthofcontinuousculturecelllinesembeddedinMebiolgel."CURRENTSCIENCE-BANGALORE-87(2004):1275-1277.
PARVEEN,NA,etal."Intraperitonealtransplantationofhepatocytesembeddedinthermoreversiblegelationpolymer(MebiolGel)inacuteliverfailureratmodel."(2008):275-280.
Kanda,Kazue,etal."Eccentricexercise-induceddelayed-onsetmusclesorenessandchangesinMarkersofmuscledamageandinflammation."Exerciseimmunologyreview19(2013).
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ProductDisclaimer
ThisproductisforR&Duseonlyandisnotintendedforhumanorotheruses.PleaseconsulttheMaterialSafetyDataSheetforinformationregardinghazardsandsafehandlingpractices.
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