ProductDescription
ECMSelect®ArrayKitUltra-36isanextracellularmatrixscreeningarray.Thisproducthasthirtysix(36)humanextracellularmatrixprotein(ECM)conditionsthataredepositedontothehydrogelsurfaceasprintedarrayspots.EachECMconditionisprintedwithnine(9)replicatespotswhereeachspothasdiameterof400µm.TheECMsarelocalizedwithineachspotwithoutcrossdiffusionfromneighboringspots,thereforeeachspotrepresentsanindependent‘well’orexperiment.
TheECMSelect®ArrayKitUltra-36containsamicroscopeglassslideprintedwiththeECMconditionscontainedwithinaprotectiveslideholderandapre-sterilizedtray.Thecomponentsaresterilizedandready-to-use.
CellsofinterestareseededontotheECMSelect®Arrayslideandallowedtobeculturedintheincubatorforthedesiredamountoftime.Cellmorphology,attachmentandgrowthcanbevisualizedusingabrightfieldmicroscope.SpecificcellularbehaviorscanbemonitoredbystainingthecellsontheslideusingspecificfluorescencebasedMarker.Fluorescencesignalcanbedetectedusingfluorescencemicroscopeimagingsystem.
TheECMSelect®ArrayKitUltra-36issimpletouseincomparisontoperformingthesameexperimentinmulti-welltissuecultureplates.Cellseeding,fixing,washingandprocessstepsaredoneinonesinglesolutionexchange,eliminatingmulti-wellsolutionhandlings.Fromstarttofinish,theoptimalECMforculturingcellscanbedeterminedinlessthan24hoursformostcelltypes.
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ProductQ&A
Becauseitiscontactprinting,itishardtodeterminethethepreciseamount.Basedonproteinstainingresult,eachspotisabout0.1-1ng.
TheremaybepossibilityofantagoNISTeffectoftwodifferentcomponent.Wehavenotseenthisveryoften,whatweusuallyseeisoneofthecomponentbyitselfdoesnotprovideadhesioneffect,butwhencombinewithanotherECM,greatlyenhancetheotherECM.
Thestiffnessis~10kPa.
MountingmediatendstodestroythepolyacrylamidehydrogelthattheECMspotsareprintedonto.Thebestmethodwehavefoundistoincubatetheentireslidefor20minuteswith4%PFApriortothestainprotocol.Theslidewascover-slippedwitha90%glycerolmediumcontainingthePPDanti-fadecompoundandsealedwithfingernailpolisharoundtheedges.
ProductReferences
ReferencesforECMSelect®:
Shaheen,NoelL.,etal."Extracellularmatrixcompositionmodulatesangiosarcomacellattachmentandproliferation."Oncoscience11-12(2017):178.
Ferreira,L.P.,V.M.Gaspar,andJ.F.Mano."DesignofSphericallyStructured3DInvitroTumorModels-AdvancesandProspects."ActaBiomaterialia(2018).
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ProductDisclaimer
ThisproductisforR&Duseonlyandisnotintendedforhumanorotheruses.PleaseconsulttheMaterialSafetyDataSheetforinformationregardinghazardsandsafehandlingpractices.
Advanced BioMatrix（简称ABM）是美国一家著名的生物公司，获得了Allergan Inc的授权(Allergan用25年时间不断完善胶原蛋白相关的产品的生产工艺)，将Allergan的专业和技术用于蛋白生产与检测，致力于为组织工程、细胞分析及细胞增殖等研究领域提供优质稳定的产品。
Advanced BioMatrix不断丰富已有产品线，目前可为三维细胞培养提供各种胶原蛋白、纤连蛋白、玻连蛋白、水性凝胶、不同粘度与分子量的透明质酸以及低代成纤维细胞等。在美国全部产品授权Sigma销售。
Advanced BioMatrix是组织培养，细胞分析和细胞增殖三维（3D）应用的生命科学领域的领导者。 我们的产品被公认为纯度，功能性和一致性的标准。
我们在生产，分离，纯化，冷冻干燥，细胞培养和蛋白质测试，粘附肽，附着因子，底物刚性和其他3D矩阵产品方面拥有丰富的专业知识。 我们的专业技术和知识正在被用来确保我们的产品质量最高，批次之间一致且易于为我们的研究客户使用。
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