Product Description
Fibronectin is a widely used broad range natural cell attachment factor. This product has been purified from human plasma where it is found as a dimer and has a size of 440-500 kDa with two similar subunits (220-250 kDa ) linked by two disulfide bonds.
Fibronectin’s primary function is related to cell adhesion and attachment to extracellular matrix. Certain domains of Fibronectin have been found to play important roles that involve interactions with collagen, heparin and other cell surface glycosaminoglycans (GAGs).
Fibronectin is ideal for coating of surfaces and is typically used at a concentration of 1-5 µg/cm2 . It is provided in user-friendly packaging for use and storage. Fibronectin is sterile filtered and is supplied as a sterile, lyophilized product.
| Parameter, Testing, and Method | Fibronectin#5080 |
| Form | Lyophilized Powder |
| Package Size | 5 mg |
| Sterilization Method | Filtration |
| Purity - HPLC | >95% |
| Formulation | 1.12 mg glycine and 0.056 mg sodium chloride per mg of fibronectin |
| Source | Human, Plasma |
| Storage Temperature | 2 to 10°C prior to reconstitution and -20°C after reconstitution |
| Shelf Life | Minimum of 6 months from date of receipt |
| Shelf Life after Reconstitution | 3 months |
| Safety | Source material found negative for infectious agents |
Directions for Use
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Coating Procedure:
Use these recommendations as guidelines todetermine the optimal coating conditions for yourculture system.
- Add 5 ml of sterile water to yield aconcentration of 1 mg/ml.
- Incubate for 30-60 minutes at 37°C to dissolve.Do not agitate.Note: Upon reconstitution, the solution maycontain a small amount of insoluble aggregatedmaterial. This phenomenon is inherent tofibronectin and does not affect productperformance.
- Further dilute the 1 mg/ml solution with PBS orcell culture medium to achieve desired workingconcentration.
- For coating of cell culture plasticware,5 µg/cm2is typical.
- Incubate for approximately 45 minutes at 15 to25°C.
- Aspirate remaining material being careful to nottouch the coated surface.
- It is possible, but not necessary, to rinse thecoated surface with PBS or medium.
- Plates are ready for use. The coated culturevessels should be used immediately and shouldnot be allowed to dry.
Product Q & A
440 kDa. The protein is a dimer of two 220 kDa identical subunits. It shows up as 220 kDa on a reduced gel, and 440 kDa on a non-reduced gel.
Product References
References for Fibronectin:
Alhussein, Ghada, et al. "A spatiotemporal characterization method for the dynamic cytoskeleton."Cytoskeleton73.5 (2016): 221-232.
Pezzoli, Daniele, et al. "Fibronectin promotes elastin deposition, elasticity and mechanical strength in cellularised collagen-based scaffolds."Biomaterials180 (2018): 130-142.
Wiley, Luke A., et al. "Generation of xeno‐free, cGMP‐compliant patient‐specific iPSCs from skin biopsy."Current protocols in stem cell biology42.1 (2017): 4A-12.
Wiley, Luke A., et al. "Using patient-specific induced pluripotent stem cells and wild-type mice to develop a gene augmentation-based strategy to treat CLN3-associated retinal degeneration."Human gene therapy27.10 (2016): 835-846.
Worthington, Kristan S., et al. "Two-photon polymerization for production of human iPSC-derived retinal cell grafts."Acta biomaterialia55 (2017): 385-395.
Li, Ling, Mizuho Fukunaga-Kalabis, and Meenhard Herlyn. "Isolation and cultivation of dermal stem cells that differentiate into functional epidermal melanocytes."Human Cell Culture Protocols. Humana Press, 2012. 15-29.
Boularaoui, Selwa Mokhtar, et al. "Efficient transdifferentiation of human dermal fibroblasts into skeletal muscle."Journal of tissue engineering and regenerative medicine12.2 (2018): e918-e936.
Product Certificate of Analysis
Safety and Documentation
Safety Data Sheet
Certificate of Origin
Product Disclaimer
This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
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