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胶原蛋白、纤连蛋白、玻连蛋白、水性凝胶、低代成纤维细胞
℡ 4000-520-616
℡ 4000-520-616
Advanced BioMatrix/Heprasil®//GS217-5EA (5 units x 1 mL)
产品编号:GS217-5EA(5unitsx1mL)
市  场 价:¥4900.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$245.00
品      牌: Advanced BioMatrix
公      司:Advanced BioMatrix ,Inc.
公司分类:
Advanced BioMatrix/Heprasil®//GS217-5EA (5 units x 1 mL)
商品介绍

ProductDescription

Heprasil®isthiol-modifiedhyaluronicacidwiththiol-modfiedheparinandisacomponentoftheHyStem®hydrogelkit.Hyaluronicacidisamajorconstituentofnativeextracellularmatrix(ECM).HeparinisalsopresentintheECMasheparansulfate.MostcellsdonotattachtoHeprasil-onlyhydrogels.HeprasilmustbeusedinconjunctionwithGelin-S®orECMproteinssuchaslaminin,collagen,orfibronectinformost3-Dcellcultureandtissue-engineeringapplications.GelationReconstitutedHeprasilremainsliquidat15to37°C.Withoutacrosslinker,Heprasilwillformahydrogelviadisulfidebondformation;however,thegelationtimeisovertwenty-fourhours.IfExtralink®isusedtocrosslinktheHeprasil,thegelationtimeisabouttwentyminuteswithnolow-temperatureorlow-pHsteps.DilutingHeprasilwithphosphate-bufferedsaline(PBS)orcell-culturemediumcanincreaseitsgelationtime.

DirectionsforUse

Heprasil®ispreparedbydissolvingthelyophilizedsolidintheDGWater(oranysterile,degassed,deionizedwater).Whenre-constituted,itwillbein1xphosphatebufferedsaline(PBS),pH~7.4.TheamountofDGWaterusedfordissolutionwilldependonthevial.

Heprasil®shouldbepreparedinthefollowingmanner:

  1. AllowtheHeprasil®vialtocometoroomtemperature.
  2. Underasepticconditions,usingasyringeandneedle,addtothevialtheamountofDGWaterindicatedonthelabel(1mLor5mL).
  3. Placethevialhorizontallyonarockerorshaker.Itwilltake<40minutesforthesolidstofullydissolve.Warmingto37°Cand/orgentlyvortexingwillspeedupdissolvingtime.Solutionswillbeclearandslightlyviscous.
  4. UsewithExtralink®orExtralink-Litetoformahydrogel.
  5. Typically,crosslinkersareusedina1:4volumeratiowiththepolymer(i.e.1.0mLofHeprasiliscrosslinkedwith0.25mLExtralink-Lite;or0.5mLHeprasilcombinedwith0.5mLGelin-S®iscrosslinkedwith0.25mLExtralink).
  6. Note:ThegelationtimewillbeshorterforgelsnotincorporatingGelin-S.HydrogelsmadeusingonlyHeprasilwillnotsupportcellattachment.UsewithGelin-Sforcellattachment.

ProductQ&A

Globularparticleslessthan75kDashouldbeabletofreelydiffusethroughaHyStemhydrogel.

WhenreconstitutedusingDGwater,thepHofeachHyStemcomponentwillbeapproximately7.4-7.6.

Oneyearfromthedateofreceipt,ifstoredproperly.

Anysterile,deionized,degassedwatercanbesubstitutedforreconstitution.However,inordertoensureaccurateandpredictabledissolutionandgelationtimes,ourDGWaterishighlyrecommended,asitisdegassed,blanketedinargon,andhasundergonevalidationtestingwitheachHyStemcomponent.

Gelin-Sprovidescellularattachmentsiteswhenincorporatedinthehydrogel.Gelin-Sisthiol-modified,denaturedcollagenI,derivedfromeitherbovineorporcinesources.Gelin-SisincludedinallHyStem-CandHyStem-HPkits.

Gelin-Shasbeenthiol-modifiedinthesamemannerasthehyaluronaninGlycosil(orHeprasil),sothatitcovalentlycrosslinkswiththeExtralinkintheHyStemhydrogels.

Yes.Peptidesthatcontainacysteineresiduecanbeused.Thecysteineresiduemustbepresentforthepeptidetobecovalentlybondedtothehydrogelsubstrate.

Yes.ECMproteins,suchaslaminin,collagen,fibronectin,orvitronectincanbenon-covalentlyincorporatedintothehydrogelpriortocrosslinking.

HyStemhydrogelsandspongesdifferinhydrationandhomogeneity.HyStemspongesaretypicallypolymerizedhydrogelsthataresubsequentlyfreeze-dried.Theresultingspongeisafibrous,meshnetworkwithporesandnichesthatenablecellstoinfiltrateandadhere.AtrueHyStemhydrogelisanencapsulatingliquidthatpolymerizesaroundsUSPendedcellsinculture.

No.ThecomplianceofthehydrogelsissetbytheamountofExtralinkcrosslinkeradded,theconcentrationofGlycosil(orHeprasil)andGelin-Sused,andtheratioofGlycosil(orHeprasil)toGelin-S.Oncethischemicalstructureofthehydrogelisfixed,itisnotalteredbyprolongedexposuretocellculturemedium.

HyStemspongescanbeterminallysterilizedbyE-beam.HyStemhydrogelshavenotyetbeenvalidatedforusewithE-beamsterilizationmethods.HyStemhydrogelsarenotterminallysterilizedbygammairrADIation.

Gelationtimeisaffectedbymultipleaspectsofthegel’scomposition.Onewaytochangethegelationtimeofahydrogelistovarytheamountofcrosslinkerused.GelswithaloweramountofExtralinkcrosslinkerwillhavealongergelationtimethanthosewithahigheramountofcrosslinker.Changingtheamountofcrosslinkerwillproduceslightchangesingelationtime.GelationtimecanbedramaticallychangedbyvaryingtheGlycosil(orHeprasil)andGelin-Sconcentrations.ConcentratedsolutionsofGlycosil(orHeprasil)andGelin-Swillcreateasolutionwithamuchshortergelationtime.ThiscaneasilybedonebyreconstitutingthecomponentsinasmallervolumeofDGWater.Alternatively,dilutingthesecomponentsinlargervolumesofDGWaterwilldramaticallyincreasethetotaltimetoformthehydrogel.

HyStemHydrogelsarevirtuallytransparentandshouldnotinterferewithmicroscopy.

HyStemhydrogelsmaygeneratemildinflammationaspartofthebody’snaturalhealingprocessinresponsetoinjury.HyStemhydrogelsdonottriggerimmuneresponsewhenusedinvivo.(Theseproductsarenotforhumanuse)

HyStemisdegradedinvivobymatrixmetalloproteinases(Collagenases)andhyaluronidases.

Trypsin,Dipase,collagenase,andhyaluronidasehavebeenusedtohelpdetachcellsfromthesurfaceorfromwithinHyStemhydrogels.

Ingeneral,theporesizeforHyStem-CandHyStem-HPhydrogelsis~17nm.

ProductApplications

Clickonthetitleofthedesiredprotocoltolearnmore:

2DCellGrowthonHyStemHydrogels

HyStem3DCellEncapsulationforCellDeliveryApplicationsGuide

HyStem3DCellEncapsulationinhydrogelsusing96-wellplates

HyStem3DCellEncapsulationinhydrogelsusingTCInserts

EnzymeDigestionofHyStemHydrogelsforRecoveryofEncapsulatedCells

FluorescentLabelingofHyStemHydrogels

CellRecoveryfromSurfaceofHyStemHydrogels

HyStemECMIncorporation

HyStemGelationTimeVariation

HyStemStiffnessVariationProtocolfor7.5mLkit

HyStemStiffnessVariationProtocolfor12.5mLkit

ProductReferences

ReferencesforHyStem®:

Gaetani,R.,etal.(2015)EpicardialapplicationofcardiacProgenitorcellsina3D-printedgelatin/hyaluronicacidpatchpreservescardiacfunctionaftermyocardialinfarction.Biomaterials61:339-348.PMID:17335875.Prestwich,G.D.,etal.(2007)3-Dcultureinsyntheticextracellularmatrices:newtissuemodelsfordrugtoxicologyandcancerdrugdiscovery.AdvEnzymeRegul47:196-207.PMID:17335875.Shu,X.Z.,etal.(2006)Synthesisandevaluationofinjectable,insitucrosslinkablesyntheticextracellularmatricesfortissueengineering.JBiomedMaterResA79:901-912.PMID:16941590.Shu,X.Z.,etal.(2003)Disulfide-crosslinkedhyaluronan-gelatinhydrogelfilms:acovalentmimicoftheextracellularmatrixforinvitrocellgrowth.Biomaterials24:3825-3834.PMID:12818555.

S.Cai,etal.(2005)Injectableglycosaminoglycanhydrogelsforcontrolledreleaseofhumanbasicfibroblastgrowthfactor.Biomaterials,26,6054-6067.D.B.Pike,etal.(2006)Heparin-regulatedreleaseofgrowthfactorsinvitroandangiogenicresponseinvivotoimplantedhyaluronanhydrogelscontainingVEGFandbFGF.Biomaterials,27,5242–5251.G.D.Prestwich,etal.(2007)3-DCultureinSyntheticExtracellularMatrices:NewTissueModelsforDrugToxicologyandCancerDrugDiscovery.invited,Adv.Enz.Res.,inpress(2007).X.Z.Shu,etal,(2006)SynthesisandEvaluationofInjectable,InSituCrosslinkableSyntheticExtracellularMatrices(sECMs)forTissueEngineering.J.BiomedMater.Res.A,79A(4),901-912.

Shu,X.Z.,etal.(2004)Insitucrosslinkablehyaluronanhydrogelsfortissueengineering.Biomaterials25:1339-1348.PMID:14643608.Mehra,T.D.,etal.(2006)Molecularstentingwithacrosslinkedhyaluronanderivativeinhibitscollagengelcontraction.JInvestDermatol126:2202-2209.PMID:16741511.Shu,X.Z.,etal.(2004)AttachmentandspreadingoffibroblastsonanRGDpeptide-modifiedinjectablehyaluronanhydrogel.JBiomedMaterResA68:365-375.PMID:14704979.Ghosh,K.,etal.(2007)CelladaptationtoaphysiologicallyrelevantECMmimicwithdifferentviscoelasticproperties.Biomaterials28:671-679.PMID:17049594.

ProductCertificateofAnalysis

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SafetyandDocumentation

CertificateofOrigin

SafetyDataSheet

ProductDisclaimer

ThisproductisforR&Duseonlyandisnotintendedforhumanorotheruses.PleaseconsulttheMaterialSafetyDataSheetforinformationregardinghazardsandsafehandlingpractices.

品牌介绍
美国Advanced BioMatrix(简称ABM) www.advancedbiomatrix.com
Advanced BioMatrix(简称ABM)是美国一家著名的生物公司,获得了Allergan Inc的授权(Allergan用25年时间不断完善胶原蛋白相关的产品的生产工艺),将Allergan的专业和技术用于蛋白生产与检测,致力于为组织工程、细胞分析及细胞增殖等研究领域提供优质稳定的产品。

Advanced BioMatrix不断丰富已有产品线,目前可为三维细胞培养提供各种胶原蛋白、纤连蛋白、玻连蛋白、水性凝胶、不同粘度与分子量的透明质酸以及低代成纤维细胞等。在美国全部产品授权Sigma销售。

Advanced BioMatrix是组织培养,细胞分析和细胞增殖三维(3D)应用的生命科学领域的领导者。 我们的产品被公认为纯度,功能性和一致性的标准。
我们在生产,分离,纯化,冷冻干燥,细胞培养和蛋白质测试,粘附肽,附着因子,底物刚性和其他3D矩阵产品方面拥有丰富的专业知识。 我们的专业技术和知识正在被用来确保我们的产品质量最高,批次之间一致且易于为我们的研究客户使用。

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