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胶原蛋白、纤连蛋白、玻连蛋白、水性凝胶、低代成纤维细胞
℡ 4000-520-616
℡ 4000-520-616
Advanced BioMatrix/SpongeCol®//5135-5EA (21 mm Diameter Discs)
市  场 价:¥7900.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$395.00
品      牌: Advanced BioMatrix
公      司:Advanced BioMatrix ,Inc.
公司分类:
Advanced BioMatrix/SpongeCol®//5135-5EA (21 mm Diameter Discs)
商品介绍

Product Description

SpongeCol®is a collagen sponge with an interpenetrating, columnar porous architecture structure.SpongeCol®contains unique columnar porous network which permits cells and nutrients to flow completely through the interpenetrating pores and provides an increased surface area for cell attachment, growth and migration.

SpongeCol®is composed of highly purified Type I collagen which best supports the attachment, proliferation, and function of cells. The collagen is lightly cross-linked for increased mechanical strength and durability for short and long term tissue culture yet is still biodegradable over the longer-term. The diameter of the pores ranges from 100 to 400 micron with the average diameter being approximately 200 microns. The collagen disc is approximately 4 mm or 21 mm in diameter and 1.5 mm thick. The sponge discs fits into a 96-well (4 mm disc) or 12-well (21 mm disc) culture plate or sanitary luer connectors for flow perfusion. Each package contains 25 (4 mm) or 5 (21 mm) collagen sponge discs.

This product is terminally sterilized and ready-to-use.

Parameter, Testing, and MethodSpongeCol®#5135
Disc Diameter4mm or 21mm
Package Size25 or 5 Sponges/Pack
Disc Thickness1.5 mm
Pore Size~200 micron diameter with a range of 100-400 micron
Endotoxin - LAL< 1.0 EU/mL
Storage TemperatureRoom Temperature
Shelf LifeMinimum of 6 months from date of receipt
Sterilization MethodIrradiation
Sterility - USP modifiedNo growth
Collagen SourcePurified Type I Collagen
Collagen Purity - Silver Staining>99%

Directions for Use

Download the full PDF forSpongeCol® 4mm discsorSpongeCol® 21mm discs,or continue reading below:

A. Preparation and Seeding:

Note: Cell attachment to the sponge is generally the most critical step in tissue culture. Temperature, pH, gas exchange and cell concentration can affect the rate and efficiency of attachment. Optimum seeding rate depends on the type of cell being cultured.

  1. Aseptically remove the sponge discs from the packaging in a laminar flow work station.
  2. Carefully place the sponges into the wells of a 12 well (21mm sponge) or 96 well (4mm sponge) tissue culture plate using a sterile instrument. Be careful not to damage the sponge as it is being transferred. It is recommended to use non-treated tissue culture plasticware.Note: Tissue-coated plasticware may need to be coated with agarose to prevent cell attachment to the plastic instead of the sponge.
  3. Suspend cells at desired concentration (1×104– 1×105cells/mL) and dispense sufficient volume of cell solution on top of the sponge placed in the well. Note: An alternative method is to suspend cells in a neutralized collagen solution (such as PureCol® type I collagen Catalog #5005-100ML or PureCol® EZ Gel Catalog #5074-35ML). Dispense collagen/cell solution on top of the sponge placed in the well.
  4. Transfer to a 37ºC incubator for about 1 – 2 hours to allow for initial cell attachment.
  5. After 1 – 2 hour, remove the plate from the incubator and check for cell attachment. Additional testing may be required to optimize the time it takes for the cells to attach to the sponge. Check the morphology of the cells. Cell adherence and spreading will dictate the time for attachment.
  6. Once the cells have adequately attached to the sponge, increase the final volume in each well to fully cover and provide adequate medium for the culture system.

B. Changing the Media:

  1. Change the media 12 to 24 hours after the initial seeding. The frequency of changes will be determined by cell type, cell attachment efficiency, pH (maintain at pH 7.0 to 7.4) utilization of medium nutrients available to cultures. More frequent medium changes may be required compared to 2D culture systems.

C. Harvesting of Cells:

Note: Protease digestion is the standard method of releasing cells from the sponges. The strength of the attachment of the cells to the collagen sponges will vary from cell line to cell line. The enzyme concentration and digestion time will vary depending upon the activity of the enzyme and the confluence of the cells. Collagenase and/or trypsin may be the preferred method.

  1. Washing the sponge with EDTA-PBS may assist the protease digestion. Add sufficient volume to cover the sponge.
  2. Aspirate the EDTA-PBS solution from the well.
  3. Add sufficient dissociation solution to the well to fully over the sponge.
  4. Transfer to a 37ºC incubator. Check for cell detachment periodically for cell detachment.
  5. Once the cells have fully detached, remove the cells and dispense in a centrifuge tube.
  6. Centrifuge the cells as require.

Product Cell Assay

Endothelial Fibroblasts at 100X magnification. Images taken after 8 days of cell culture within SpongeCol®. Cells stained with Endothelial RSP.

Product References

References for SpongeCol®:

1. Mak, W. C., et al. “Thermo-Rheological Responsive Microcapsules for Time-Dependent Controlled Release of Human Mesenchymal Stromal Cells.”Biomater. Sci., vol. 5, no. 11, 2017, pp. 2241–2250., doi:10.1039/c7bm00663b.

2. Udhayakumar, Sivalingam, et al. “l-Arginine Intercedes Bio-Crosslinking of a Collagen–Chitosan 3D-Hybrid Scaffold for Tissue Engineering and Regeneration: in Silico, in Vitro, and in Vivo Studies.”RSC Advances, vol. 7, no. 40, 2017, pp. 25070–25088., doi:10.1039/c7ra02842c.

3. Ferreira, L.p., et al. “Design of Spherically Structured 3D in Vitro Tumor Models -Advances and Prospects.”Acta Biomaterialia, 2018, doi:10.1016/j.actbio.2018.05.034.

4. Hatsell, Sarah J., et al. “ACVR1 R206H Receptor Mutation Causes Fibrodysplasia Ossificans Progressiva by Imparting Responsiveness to Activin A.”Science Translational Medicine, vol. 7, no. 303, Feb. 2015, doi:10.1126/scitranslmed.aac4358.

5. Anton-Sales, I., Beekmann, U., Laromaine, A., Roig, A. & Kralisch, D. Opportunities of Bacterial Cellulose to Treat Epithelial Tissues.Current Drug Targets20,808–822 (2019).

Product Certificate of Analysis

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Product Videos

link to library blog - SpongeCol<sup>®</sup> Collagen Sponge
SpongeCol® Collagen Sponge

Video

link to library blog - PureCol<sup>®</sup> EZ Gel - Easy 3D Collagen Gels
PureCol® EZ Gel - Easy 3D Collagen Gels

Video

link to library blog - Seeding Collagen Gels with Cells
Seeding Collagen Gels with Cells

Video

See More

Safety and Documentation

Safety Data Sheet

Certificate of Origin 4mm

Certificate of Origin 21mm

Declaration of Material Source

Product Disclaimer

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

品牌介绍
美国Advanced BioMatrix(简称ABM) www.advancedbiomatrix.com
Advanced BioMatrix(简称ABM)是美国一家著名的生物公司,获得了Allergan Inc的授权(Allergan用25年时间不断完善胶原蛋白相关的产品的生产工艺),将Allergan的专业和技术用于蛋白生产与检测,致力于为组织工程、细胞分析及细胞增殖等研究领域提供优质稳定的产品。

Advanced BioMatrix不断丰富已有产品线,目前可为三维细胞培养提供各种胶原蛋白、纤连蛋白、玻连蛋白、水性凝胶、不同粘度与分子量的透明质酸以及低代成纤维细胞等。在美国全部产品授权Sigma销售。

Advanced BioMatrix是组织培养,细胞分析和细胞增殖三维(3D)应用的生命科学领域的领导者。 我们的产品被公认为纯度,功能性和一致性的标准。
我们在生产,分离,纯化,冷冻干燥,细胞培养和蛋白质测试,粘附肽,附着因子,底物刚性和其他3D矩阵产品方面拥有丰富的专业知识。 我们的专业技术和知识正在被用来确保我们的产品质量最高,批次之间一致且易于为我们的研究客户使用。

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