ProductDescription
CDH13geneencodesamemberofthecadherinsuperfamily.TheencodedproteinislocalizedtothesurfaceofthecellmembraneandisanchoredbyaGPImoiety,ratherthanbyatransmembranedomain.Theproteinlacksthecytoplasmicdomaincharacteristicofothercadherins,andsoisnotthoughttobeacell-celladhesionglycoprotein.Thisproteinactsasanegativeregulatorofaxongrowthduringneuraldifferentiation.Italsoprotectsvascularendothelialcellsfromapoptosisduetooxidativestress,andisassociatedwithresistancetoatherosclerosis.Thegeneishypermethylatedinmanytypesofcancer.
Full-lengthextracellulardomainofhumanCDH13gene(139-693aa)wasconstructedwith31N-terminalT7/HistagandexpressedinE.coliasinclusionbodies.Thefinalproductwasrefoldedusingourunique“temperatureshiftinclusionbodyrefolding”technologyandchromatographicallypurified.
| Parameter,Testing,andMethod | CDH13#5125 |
| Quantity | 0.1mg |
| Volume | 0.2mL |
| Concentration | 0.5mg/mL |
| Purity-SDSPAGEElectrophoresis | >90% |
| Formulation | FOrmulatedin20mMpH8.0TRIS-HCLBuffer,withproprietaryformulationofNaCl,KCl,EDTA,L-Arginine,DTTandGlycerol |
| Form | Solution |
| ProductionType | Recombinant-E.Coli |
| StorageTemperature | -20°C |
| ShelfLife | Minimumof6monthsfromdateofreceipt |
| SterilizationMethod | Filtration |
| CellAssay | Pass |
| Sterility-USPmodified | NoGrowth |
| AccessionNumber | NP_001248 |
| RecombinantProteinSequence | MASMTGGQQMGRGHHHHHHGNLYFQGGEFELSIVVSPILIPENQRQPFPRDVGKVVDSDRPERSKFRLTGKGVDQEPKGIFRINENTGSVSVTRTLDREVIAVYQLFVETTDVNGKTLEGPVPLEVIVIDQNDNRPIFREGPYIGHVMEGSPTGTTVMRMTAFDADDPATDNALLRYNIRQQTPDKPSPNMFYIDPEKGDIVTVVSPALLDRETLENPKYELIIEAQDMAGLDVGLTGTATATIMIDDKNDHSPKFTKKEFQATVEEGAVGVIVNLTVEDKDDPTTGAWRAAYTIINGNPGQSFEIHTNPQTNEGMLSVVKPLDYEISAFHTLLIKVENEDPLVPDVSYGPSSTATVHITVLDVNEGPVFYPDPMMVTRQEDLSVGSVLLTVNATDPDSLQHQTIRYSVYKDPAGWLNINPINGTVDTTAVLDRESPFVDNSVYTALFLAIDSGNPPATGTGTLLITLEDVNDNAPFIYPTVAEVCDDAKNLSVVILGASDKDLHPNTDPFKFEIHKQAVPDKVWKISKINNTHALVSLLQNLNKANYNLPIMVTDSGKPPMTNITDLRVQVCSCRNSKVDCNAAG |
DirectionsforUse
DownloadthefullPDFversionorcontinuereADIngbelow:
Usetheserecommendationsasguidelinestodeterminetheoptimalcoatingconditionsforyourculturesystem.
- ThawCDH13anddilutetodesiredconcentrationusingserum-freemediumorPBS.Thefinalsolutionshouldbesufficientlydilutesothatthevolumeaddedcoversthesurfaceevenly.
- Addappropriateamountofdilutedmaterialtoculturesurface.
- Incubateatroomtemperatureforapproximately1–2hours.
- Aspirateremainingmaterial.
- RinseplatescarefullywithdH2O–avoidscratchingbottomsurfaceofplates.
- Platesarereadyforuse.Theymayalsobestoredat2-8°Cdamporairdriedifsterilityismaintained.
Coatingthisrecombinantproteinat1-10ug/well(6wellplate)inneuronalcellspecificmediumcanbeusedfor1)humanneuronalcellandvascularendothelialcell/receptorinteractionstudyor2)forhumanneuronalandvascularendothelialcell/receptorinteractioninvitro.
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ProductDisclaimer
ThisproductisforR&Duseonlyandisnotintendedforhumanorotheruses.PleaseconsulttheMaterialSafetyDataSheetforinformationregardinghazardsandsafehandlingpractices.
Advanced BioMatrix（简称ABM）是美国一家著名的生物公司，获得了Allergan Inc的授权(Allergan用25年时间不断完善胶原蛋白相关的产品的生产工艺)，将Allergan的专业和技术用于蛋白生产与检测，致力于为组织工程、细胞分析及细胞增殖等研究领域提供优质稳定的产品。
Advanced BioMatrix不断丰富已有产品线，目前可为三维细胞培养提供各种胶原蛋白、纤连蛋白、玻连蛋白、水性凝胶、不同粘度与分子量的透明质酸以及低代成纤维细胞等。在美国全部产品授权Sigma销售。
Advanced BioMatrix是组织培养，细胞分析和细胞增殖三维（3D）应用的生命科学领域的领导者。 我们的产品被公认为纯度，功能性和一致性的标准。
我们在生产，分离，纯化，冷冻干燥，细胞培养和蛋白质测试，粘附肽，附着因子，底物刚性和其他3D矩阵产品方面拥有丰富的专业知识。 我们的专业技术和知识正在被用来确保我们的产品质量最高，批次之间一致且易于为我们的研究客户使用。
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