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胶原蛋白、纤连蛋白、玻连蛋白、水性凝胶、低代成纤维细胞
℡ 4000-520-616
℡ 4000-520-616
Advanced BioMatrix/Peptite-2000®//5020-5MG
产品编号:5020-5MG
市  场 价:¥5600.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$280.00
品      牌: Advanced BioMatrix
公      司:Advanced BioMatrix ,Inc.
公司分类:
Advanced BioMatrix/Peptite-2000®//5020-5MG
商品介绍

Product Description

PEPTITE-2000®is a synthetic peptide containing the RGD cell attachment sequence found in fibronectin, vitronectin and many other matrix and serum proteins. This RGD peptide has been specifically engineered to spontaneously adhere to a variety of surfaces such as polystyrene (either tissue culture treated or non-treated), hollow fibers made of cellulose, glass, polycarbonate membranes and many other materials. This binding is mediated via a hydroscopic C-terminal sequence. The RGD motif is present at the N-terminal end of the peptide, allowing for optimal cell attachment via integrin receptors.

PEPTITE-2000®can be substituted as the primary cell attachment component in a chemically defined animal component-free culture system.

Parameter, Testing, and Method

PEPTITE-2000®Catalog # 5020

Form

Powder

Package Size

5 mg

Sterilization Method

Irradiation

Peptide Sequence

Ac-Gly-D-Arg-Gly-Asp-Ile-Pro-Ala-Ser-Ser-Lys-Gly-Gly-Gly-Gly-Ser-D-Arg-Leu-Leu- Leu-Leu- Leu-Leu-D-Arg-NH2

Counter Ion

Acetate

Purity Confirmed by RP-HPLC

>95%

Identity Confirmed by Mass Spectrometry

Characteristic

Cell Attachment Assay

Pass

Sterility- USP modified

No growth

Storage Temperature

-20°C

Shelf Life

Minimum of 6 months from date of receipt

PEPTITE-2000®is supplied in sterile 5 mg quantities.

Directions for Use

Download the full PDF version or continue reading below:

Use these recommendations as guidelines todetermine the optimal coating conditions for your culture system. To maintain sterility, perform all operations in a laminar flow hood. Two options are provided:

Procedure A

  1. Remove cap and add 5 mL of serum-free medium or PBS to the bottle.
  2. Replace cap and vortex contents vigorously. Ensure that the PEPTITE-2000®is completely solubilized. The solution will remain slightly hazy.
  3. Transfer desired volume of solution from the bottle to a dilution vessel. Dilute to desired concentration using serum-free medium or PBS. A typical working concentration may range from 0.1 to 10 µg/mL.
  4. Add appropriate amount of diluted material to culture surface.
  5. Incubate at room temperature or 37°C, covered, for 1-2 hours.
  6. After incubation, aspirate remaining material.
  7. Rinse plates carefully with dH2O– avoid scratching bottom surface of plates.
  8. Plates are ready for use. They may also be stored at 2-8°C damp or air dried if sterility is maintained.
  9. Store remaining solubilized PEPTITE-2000®at 2 to 10°C.

Additional note: Include divalent cations (Calcium, Magnesium, or Manganese) in cell attachment solution to obtain optimum cell binding.

Procedure B

  1. Remove cap and add 5 mL of sterile 70% Ethanol
  2. Replace cap and vortex contents. Ensure that the PEPTITE-2000®is completely solubilized.
  3. Transfer desired volume of solution from the bottle to a dilution vessel. Dilute to the desired concentration using 70% Ethanol. Concentrations from 0.1 to 10 ug/mL should be tested.
  4. Add appropriate amount of diluted material to culture surface.
  5. Leave the coated container, uncovered, in a laminar flow hood until the wells are dry.
  6. Rinse plates carefully with dH2O– avoid scratching bottom surface of plates.
  7. Plates are ready for use.
  8. Store remaining solubilized PEPTITE-2000®at 2-10°C.

Product References

References for Peptite-2000®:

Geuss, Laura R., Scott Gorthey, and Laura J. Suggs. "Magnetic Attraction of Paramagnetic Beads Influences Lineage Commitment in Embryoid Bodies."

Geuss, Laura R., et al. "Paramagnetic beads and magnetically mediated strain enhance cardiomyogenesis in mouse embryoid bodies."PloS one9.12 (2014): e113982.

Sanchez‐Rodriguez, Sandra P., et al. "Plasmonic activation of gold nanorods for remote stimulation of calcium signaling and protein expression in HEK 293T cells."Biotechnology and bioengineering113.10 (2016): 2228-2240.

Saito, Masaya, et al. "Intestinal epithelial cells with impaired autophagy lose their adhesive capacity in the presence of TNF-α."Digestive diseases and sciences57.8 (2012): 2022-2030.

Product Certificate of Analysis

No result for .

Safety and Documentation

Safety Data Sheet

Certificate of Origin

Product Disclaimer

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.

品牌介绍
美国Advanced BioMatrix(简称ABM) www.advancedbiomatrix.com
Advanced BioMatrix(简称ABM)是美国一家著名的生物公司,获得了Allergan Inc的授权(Allergan用25年时间不断完善胶原蛋白相关的产品的生产工艺),将Allergan的专业和技术用于蛋白生产与检测,致力于为组织工程、细胞分析及细胞增殖等研究领域提供优质稳定的产品。

Advanced BioMatrix不断丰富已有产品线,目前可为三维细胞培养提供各种胶原蛋白、纤连蛋白、玻连蛋白、水性凝胶、不同粘度与分子量的透明质酸以及低代成纤维细胞等。在美国全部产品授权Sigma销售。

Advanced BioMatrix是组织培养,细胞分析和细胞增殖三维(3D)应用的生命科学领域的领导者。 我们的产品被公认为纯度,功能性和一致性的标准。
我们在生产,分离,纯化,冷冻干燥,细胞培养和蛋白质测试,粘附肽,附着因子,底物刚性和其他3D矩阵产品方面拥有丰富的专业知识。 我们的专业技术和知识正在被用来确保我们的产品质量最高,批次之间一致且易于为我们的研究客户使用。

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