PEPTITE-2000®is a synthetic peptide containing the RGD cell attachment sequence found in fibronectin, vitronectin and many other matrix and serum proteins. This RGD peptide has been specifically engineered to spontaneously adhere to a variety of surfaces such as polystyrene (either tissue culture treated or non-treated), hollow fibers made of cellulose, glass, polycarbonate membranes and many other materials. This binding is mediated via a hydroscopic C-terminal sequence. The RGD motif is present at the N-terminal end of the peptide, allowing for optimal cell attachment via integrin receptors.
PEPTITE-2000®can be substituted as the primary cell attachment component in a chemically defined animal component-free culture system.
Parameter, Testing, and Method | PEPTITE-2000®Catalog # 5020 |
Form | Powder |
Package Size | 5 mg |
Sterilization Method | Irradiation |
Peptide Sequence | Ac-Gly-D-Arg-Gly-Asp-Ile-Pro-Ala-Ser-Ser-Lys-Gly-Gly-Gly-Gly-Ser-D-Arg-Leu-Leu- Leu-Leu- Leu-Leu-D-Arg-NH2 |
Counter Ion | Acetate |
Purity Confirmed by RP-HPLC | >95% |
Identity Confirmed by Mass Spectrometry | Characteristic |
Cell Attachment Assay | Pass |
Sterility- USP modified | No growth |
Storage Temperature | -20°C |
Shelf Life | Minimum of 6 months from date of receipt |
PEPTITE-2000®is supplied in sterile 5 mg quantities.
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Use these recommendations as guidelines todetermine the optimal coating conditions for your culture system. To maintain sterility, perform all operations in a laminar flow hood. Two options are provided:
Procedure A
Additional note: Include divalent cations (Calcium, Magnesium, or Manganese) in cell attachment solution to obtain optimum cell binding.
Procedure B
References for Peptite-2000®:
Geuss, Laura R., Scott Gorthey, and Laura J. Suggs. "Magnetic Attraction of Paramagnetic Beads Influences Lineage Commitment in Embryoid Bodies."
Geuss, Laura R., et al. "Paramagnetic beads and magnetically mediated strain enhance cardiomyogenesis in mouse embryoid bodies."PloS one9.12 (2014): e113982.
Sanchez‐Rodriguez, Sandra P., et al. "Plasmonic activation of gold nanorods for remote stimulation of calcium signaling and protein expression in HEK 293T cells."Biotechnology and bioengineering113.10 (2016): 2228-2240.
Saito, Masaya, et al. "Intestinal epithelial cells with impaired autophagy lose their adhesive capacity in the presence of TNF-α."Digestive diseases and sciences57.8 (2012): 2022-2030.
Safety Data Sheet
Certificate of Origin
This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
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