Directions for Use

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Use these recommendations as guidelines to determine the optimal coating conditions for your culture system. To maintain sterility, perform all operations in a laminar flow hood.

1. A typical working concentration is 0.1 mg/ml. To achieve a 0.1 mg/ml concentration, add 50 ml of tissue culture grade water or PBS to the 50 ml bottle. If a different concentration is desired, transfer desired volume of solution from the bottle to a dilution vessel. Dilute to desired concentration using tissue culture grade water or PBS.
2. Add appropriate amount of diluted material to culture surface. Typically, 1 ml per 25 cm²is used. Rock gently to ensure uniform coating of culture surface.

Option A:

Allow to incubate for 60 minutes at room temperature, remove excess solution by aspiration.

3. Thoroughly rinse surface with tissue culture grade water and aspirate rinse water.
4. Incubate and allow to dry at room temperature or 37°C, covered, for at least 2 hours. Coated cultureware can be stored for up to 1 week. Go to Step 9

Option B:

6. Allow to incubate overnight at room temperature, remove excess solution by aspiration.
7. Thoroughly rinse surface with tissue culture grade water and aspirate rinse water.
8. Incubate and allow to dry at room temperature or 37°C, covered, for at least 2 hours. Coated cultureware can be stored for up to 1 week.
9. Introduce medium and cells to the culture surface.

Store remaining Poly-D-Lysine solution at -10 to 30°C

Product References

References for Poly-D-Lysine:

Albuquerque, Cristóvão, et al. "Preparation of coverslips for neuronal cultures."Cold Spring Harbor Protocols2009.8 (2009): pdb-prot5272.

Unsain, Nicolas, et al. "Production and isolation of axons from sensory neurons for biochemical analysis using porous filters."JoVE (Journal of Visualized Experiments)89 (2014): e51795.

Li, Ling, Mizuho Fukunaga-Kalabis, and Meenhard Herlyn. "Isolation, characterization, and differentiation of human multipotent dermal stem cells."Skin Stem Cells. Humana Press, Totowa, NJ, 2013. 235-246.

Baik, Matthew, et al. "Identification of invadopodia by TKS5 staining in human cancer lines and patient tumor samples."MethodsX6 (2019): 718-726.

Tammia, Markus, et al. "Egr2 overexpression in Schwann cells increases myelination frequency in vitro."Heliyon4.11 (2018): e00982.

Boularaoui, Selwa Mokhtar, et al. "Efficient transdifferentiation of human dermal fibroblasts into skeletal muscle."Journal of tissue engineering and regenerative medicine12.2 (2018): e918-e936.

Johnstone, Aaron D., et al. "Developmental axon degeneration requires TRPV1-dependent Ca2+ influx."eNeuro6.1 (2019).

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Safety and Documentation

Safety Data Sheet

Certificate of Origin

Product Disclaimer

This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.