Product Description
Poly-L-Lysineis a synthetic amino acid chain that is positively charged having one hydrobromide per unit of Lysine.Poly-L-Lysineis widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces. This molecule has been used to culture a wide variety of cell types. Certain cell types secrete proteases, which can digestPoly-L-Lysine. For those cell types, Poly-D-Lysine, Catalog # 5049 should be used.
The molecular weight ofPoly-L-Lysinecan vary significantly with lower molecular weight (30,000 Da) being less viscous and higher molecular weight (>300,000 Da) having more binding sites per molecule. This product’s molecular weight ranges from 70,000 to 150,000 Da yielding a solution viscosity for easy handling while providing sufficient binding sites for cell attachment.
Poly-L-Lysinesurface coatings are designed to improve cell attachment, growth and differentiation of many cell types.
Coated surfaces will often improve cell attachment in reduced or serum-free conditions. This product is supplied in a sterile 50 ml package size at a concentration of 0.1 mg/ml.
| Parameter, Testing, and Method | Poly-L-Lysine #5048 |
| Form | Solution |
| Package Size | 50 mL |
| Storage Temperature | 2-10°C |
| Sterilization Method | Filtration |
| Molecular Weight | 70,000 - 150,000 Da |
| Sterility - USP modified | No Growth |
| Cell Attachment Assay | Pass |
| Source | Synthetic |
| Shelf Life | Minimum of 6 months from date of receipt |
| Concentration | 0.1 mg/mL |
Directions for Use
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Use these recommendations as guidelines todetermine the optimal coating conditions for your culture system. To maintain sterility, perform all operations in a laminar flow hood.
A typical working concentration is 0.1 mg/mL. If a different concentration is desired, transfer desired volume of solution from the bottle to a dilution vessel. Dilute to desired concentration using tissue culture grade water or PBS.
- Add appropriate amount of diluted material to culture surface. Typically, 1 ml per 25 cm2is used. Rock gently to ensure uniform coating of culture surface.
- After 5 minutes, remove excess solution by aspiration.
- Thoroughly rinse surface with tissue culture grade water.
- Incubate and allow to dry at room temperature or 37°C, covered, for at least 2 hours.
- Introduce medium and cells to the culture surface.
- Store remaining Poly-L-Lysine at 2 to 10°C.
Product References
References for Poly-L-Lysine:
Påhlman, Lisa I., et al. "Hypoxia down-regulates expression of secretory leukocyte protease inhibitor in bronchial epithelial cells via TGF-β1."BMC pulmonary medicine15.1 (2015): 19.
Li, Yun, et al. "Imaging pHluorin-tagged receptor insertion to the plasma membrane in primary cultured mouse neurons."JoVE (Journal of Visualized Experiments)69 (2012): e4450.
Morand, David-Nicolas, et al. "Active nanofibrous membrane effects on gingival cell inflammatory response."Materials8.10 (2015): 7217-7229.
Thuault, Sylvie, et al. "The RhoE/ROCK/ARHGAP25 signaling pathway controls cell invasion by inhibition of Rac activity."Molecular biology of the cell27.17 (2016): 2653-2661.
Popielarski, Marcin, et al. "The role of Protein Disulfide Isomerase and thiol bonds modifications in activation of integrin subunit alpha11."Biochemical and biophysical research communications495.2 (2018): 1635-1641.
Wu, Kun Lieh, et al. "Effects of culturing media on hepatocytes differentiation using Volvox sphere as co-culturing vehicle."Biochemical and biophysical research communications458.3 (2015): 620-625.
Santio, Niina M., et al. "The PIM1 kinase promotes prostate cancer cell migration and adhesion via multiple signalling pathways."Experimental cell research342.2 (2016): 113-124.
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Safety and Documentation
Safety Data Sheet
Certificate of Origin
Product Disclaimer
This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
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