Product Description
Poly-L-Lysine is a synthetic amino acid chain that is positively charged and widely used as a coating to enhance cell attachment and adhesion to both plasticware and glass surfaces.
The molecular weight of Poly-L-Lysine can vary significantly with lower molecular weight (30,000 Da) being less viscous and higher molecular weight (>300,000 Da) having more binding sites per molecule. This product’s molecular weight ranges from 70,000 to 150,000 Da.
Poly-L-Lysine is supplied in a 50 ml amber bottle containing 5 mg of Poly-L-Lysine. The product has been sterilized and is ready-to-use after proper addition of cell culture water.
| Parameter, Testing, and Method | Poly-L-Lysine #5173 |
| Form | Powder |
| Package Size | 5 mg |
| Storage Temperature | -20°C |
| Sterilization Method | Irradiation |
| Molecular Weight | 70,000 - 150,000 Da |
| Sterility - USP modified | No Growth |
| Cell Attachment Assay | Pass |
| Source | Synthetic |
| Shelf Life | Minimum of 6 months from date of receipt |
Directions for Use
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Use these recommendations as guidelines to determine the optimal coating conditions for your culture system. To maintain sterility, perform all operations in a laminar flow hood.
- A typical working concentration is 0.1 mg/ml. To achieve a 0.1 mg/ml concentration, add 50 ml of tissue culture grade water or PBS to the 50 ml bottle. If a different concentration is desired, transfer desired volume of solution from the bottle to a dilution vessel. Dilute to desired concentration using tissue culture grade water or PBS.
- Add appropriate amount of diluted material to culture surface. Typically, 1 ml per 25 cm2is used. Rock gently to ensure uniform coating of culture surface.
Option A:
- Allow to incubate for 60 minutes at room temperature, remove excess solution by aspiration.
- Thoroughly rinse surface with tissue culture grade water and aspirate rinse water.
- Incubate and allow to dry at room temperature or 37°C, covered, for at least 2 hours. Coated cultureware can be stored for up to 1 week. Go to Step 9
Option B:
- Allow to incubate overnight at room temperature, remove excess solution by aspiration.
- Thoroughly rinse surface with tissue culture grade water and aspirate rinse water.
- Incubate and allow to dry at room temperature or 37°C, covered, for at least 2 hours. Coated cultureware can be stored for up to 1 week.
- Introduce medium and cells to the culture surface.
Store remaining Poly-L-Lysine solution at -10 to 30°C
Product References
References for Poly-L-Lysine:
Påhlman, Lisa I., et al. "Hypoxia down-regulates expression of secretory leukocyte protease inhibitor in bronchial epithelial cells via TGF-β1."BMC pulmonary medicine15.1 (2015): 19.
Li, Yun, et al. "Imaging pHluorin-tagged receptor insertion to the plasma membrane in primary cultured mouse neurons."JoVE (Journal of Visualized Experiments)69 (2012): e4450.
Morand, David-Nicolas, et al. "Active nanofibrous membrane effects on gingival cell inflammatory response."Materials8.10 (2015): 7217-7229.
Thuault, Sylvie, et al. "The RhoE/ROCK/ARHGAP25 signaling pathway controls cell invasion by inhibition of Rac activity."Molecular biology of the cell27.17 (2016): 2653-2661.
Popielarski, Marcin, et al. "The role of Protein Disulfide Isomerase and thiol bonds modifications in activation of integrin subunit alpha11."Biochemical and biophysical research communications495.2 (2018): 1635-1641.
Wu, Kun Lieh, et al. "Effects of culturing media on hepatocytes differentiation using Volvox sphere as co-culturing vehicle."Biochemical and biophysical research communications458.3 (2015): 620-625.
Santio, Niina M., et al. "The PIM1 kinase promotes prostate cancer cell migration and adhesion via multiple signalling pathways."Experimental cell research342.2 (2016): 113-124.
Product Certificate of Analysis
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Safety and Documentation
Safety Data Sheet
Certificate of Origin
Product Disclaimer
This product is for R&D use only and is not intended for human or other uses. Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
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