ProductDescription
CollagenHybridizingPeptides(CHP)conjugatesfromAdvancedBiomatrixarelabeledwith5-FAM(F-CHP,CatalogNo.5264-60UG)/sulfo-Cyanine3(R-CHP,CatalogNo.5276-60UG)forfluorescencedetection,orbiotin(B-CHP,CatalogNo.5265-UG)foravidin/streptavidin-mediateddetection.
Thetriplehelixisthehallmarkproteinstructureofcollagen.Duringtissueremodeling,thetriplehelicalcollagenmoleculesaredegradedbyspecificproteases(e.g.,MMPorcathepsinK)andbecomeunfoldedatbodytemperature.TheCollagenHybridizingPeptide(CHP)isasyntheticpeptidethatcanspecificallybindtosuchdenaturedcollagenstrandsthroughhydrogenbonding,bothinhistology,invivo,andinvitro(3Dcellculture).BysharingthestructuralmotifandtheGly-X-Yrepeatingsequenceofnaturalcollagen,CHPhasastrongcapABIlitytohybridizewithdenaturedcollagenstrands,inafashionthatissimilartoaDNAfragmentannealingtoitscomplimentaryDNAstrandduringPCR.CHPisanextremelyspecificprobeforunfoldedcollagenmolecules:ithasnegligIBLeaffinitytointactcollagenmoleculesduetothelackofbindingsites;itisalsoinerttowardsnon-specificbindingbecauseofitsneutralandhydrophilicnature.
| Parameter,Testing,andMethod | CHP5-FAM#5264 |
| Specialty | Straight-forwardfluore-scencedetectioningreen |
| Formula | C135H175N31O45 |
| MolecularWeight | 2952.01g/mol |
| Ex/Em | 494nm/512nm |
| Synonym | CollagenMimeticPeptide,CMP |
| Solubility | AqueousBuffers |
| Storage | -20°Caspowderforlongtermstorage.4°Cafterreconstitutioninwater.Protectfromlight. |
| Source | Synthetic |
| ShelfLife | Minimumof6monthsfromdateofreceipt |
Storage/Stability:
Theproductshipsonambient.Storetheproductat-20°C.
FeaturesofCHPreagents
- Moreinformative,reliableandconvenientthanzymography,DQcollagen,SHG,andTEM
- Highaffinityandunparalleledspecificitytocollagenwithessentiallynononspecificbinding
- Applicabletodenaturedcollagensofallsubtypesandfromallspecies;bindingrelyingoncollagen’ssecondarystructureinsteadofspecificepitopes
- Suitableforbothfrozenandparaffin-embeddedsectionswithnoneedforantigenretrieval
- Anon-antibodyapproachwithnospeciesrestrictions,compatibleforco-stainingwithanyantibody
- Smallsize(2%ofIgGbyMW)enablingfaciletissuepenetrationduringwholetissuestaining(withnoneedforsectioning)
- Stableinsolutionunder4°C,eliminatingtheneedtoaliquotforstorage
DirectionsforUse
DownloadtheDirectionsforUsePDF
ProductQ&A
TheCHPisfairlystable.Therefore,itispossibletoreuseadilutesolutionthathasbeenheatedalready,althoughtheintensityofthestainmaydecreaseandresultsmaynotbeasoptimal.Toreusethedilutesolution,itneedstobeheatedto80Candcooleddownagainimmediatelypriortoreuse.
ProductReferences
ReferencesforCollagenHybridizingPeptides:
1.Targetingandmimickingcollagensviatriplehelicalpeptideassemblies.CurrentOpinioninChemicalBIOLOGy,2013,17,968–975.
2.Insituimagingoftissueremodelingwithcollagenhybridizingpeptides.ACSNano,2017,11,9825–9835.
3.Targetingcollagenstrandsbyphoto-triggeredtriple-helixhybridization.ProceedingsoftheNationalAcademyofSciencesoftheUnitedStatesofAmerica,2012,109,14767–14772.
4.Visualizingcollagenproteolysisbypeptidehybridization:From3Dcellculturetoinvivoimaging.Biomaterials,2018,183,67–76.
5.Directdetectionofcollagenousproteinsbyfluorescentlylabeledcollagenmimeticpeptides.BioconjugateChemistry,2013,24,9–16.
6.Molecularleveldetectionandlocalizationofmechanicaldamageincollagenenabledbycollagenhybridizingpeptides.NatureCommunications,2017,8,14913,doi:10.1038/ncomms14913.
7.Molecularassessmentofcollagendenaturationindecellularizedtissuesusingacollagenhybridizingpeptide.ActaBiomaterialia,2017,53,268–278.
ProductCertificateofAnalysis
SafetyandDocumentation
SafetyDataSheet
CertificateofOrigin
ProductDisclaimer
ThisproductisforR&Duseonlyandisnotintendedforhumanorotheruses.PleaseconsulttheMaterialSafetyDataSheetforinformationregardinghazardsandsafehandlingpractices.
Advanced BioMatrix（简称ABM）是美国一家著名的生物公司，获得了Allergan Inc的授权(Allergan用25年时间不断完善胶原蛋白相关的产品的生产工艺)，将Allergan的专业和技术用于蛋白生产与检测，致力于为组织工程、细胞分析及细胞增殖等研究领域提供优质稳定的产品。
Advanced BioMatrix不断丰富已有产品线，目前可为三维细胞培养提供各种胶原蛋白、纤连蛋白、玻连蛋白、水性凝胶、不同粘度与分子量的透明质酸以及低代成纤维细胞等。在美国全部产品授权Sigma销售。
Advanced BioMatrix是组织培养，细胞分析和细胞增殖三维（3D）应用的生命科学领域的领导者。 我们的产品被公认为纯度，功能性和一致性的标准。
我们在生产，分离，纯化，冷冻干燥，细胞培养和蛋白质测试，粘附肽，附着因子，底物刚性和其他3D矩阵产品方面拥有丰富的专业知识。 我们的专业技术和知识正在被用来确保我们的产品质量最高，批次之间一致且易于为我们的研究客户使用。
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